Research
| Title： | A novel method for evaluating the efficacy of pharmaceuticals against the in vivo stage of Ichthyophthirius multifiliis in fish |
|---|---|
| First author： | Hu, Guang-Ran; Zeng, Qing-Wen; Huang, Ke; Zou, Hong; Li, Wen-Xiang; Wu, Shan-Gong; Wang, Gui-Tang; Li, Ming |
| Journal： | METHODSX |
| Years： | 2025 |
| DOI： | 10.1016/j.mex.2025.103480 |
| Abstract： | This study presents a description of a novel apparatus for evaluating the efficacy of pharmaceuticals against the in vivo stage of the fish parasite Ichthyophthirius multifiliis. The apparatus comprises a transparent acrylic rectangular cuboid, a metal wire mesh, and a gridded black acrylic bottom plate. Fallen trophonts are caught on the bottom plate and are separated from the fish by the mesh. The efficacy of a pharmaceutical can be assessed by adding it to the aquarium and subsequently counting the number of live tomonts and differntiating rate on the bottom plate. This device facilitates observation and enhances the precision and accuracy of pharmaceutical evaluation experiments. |
