Research
| Title： | Determination of Multiple Metal Elements in Cyanobacteria Culture Media by Metal Capture/Enrichment Hyphenated with Inductively Coupled Plasma Mass Spectrometry |
|---|---|
| First author： | Men, Jun; Wang, Siyu; Zheng, Lingling; Zhou, Fang; Qiao, Zhixian; Chai, Xiaocui; Ge, Feng; Zuo, Yanxia |
| Journal： | SEPARATIONS |
| Years： | 2025 |
| DOI： | 10.3390/separations12110310 |
| Abstract： | To address the challenges posed by extensive sample pretreatment and significant matrix interference in conventional metal quantification methods for cyanobacterial culture media, an automated online metal capture and enrichment system was developed and integrated with inductively coupled plasma mass spectrometry (ICP-MS). This system enabled the simultaneous determination of nine metal elements-Cd, Pb, V, Mn, Fe, Co, Ni, Cu, and Zn-within the culture medium. Through systematic optimization and validation, the method demonstrated exceptional analytical performance: calibration curves for all analytes exhibited correlation coefficients (r) exceeding 0.999; repeatability tests yielded relative standard deviations (RSD) below 3% (n = 6); and recoveries at low, medium, and high spike levels ranged from 93.98% to 108.70%. The procedure is characterized by simplicity, high automation, low detection limits, and robust accuracy, making it an effective platform for multi-element contamination monitoring and metal metabolic studies in cyanobacterial cultivation. This approach holds significant potential for applications in algal resource utilization and environmental restoration. |
