Research
| Title： | Functional characterization of the plastidial enzyme GPAT in microalgae and its potential role in lipid biosynthesis |
|---|---|
| First author： | Chen, Hong; Ma, Haiyan; Yu, Lihua; Zou, Song; Li, Yanhua; Lu, Yuanchen; Yang, Mingkun; Ge, Feng; Yuan, Li; Zeng, Xiaoli; Zhang, Cheng-Cai; Yoon, Kangsup; Han, Danxiang; Hu, Qiang |
| Journal： | PLANT PHYSIOLOGY |
| Years： | 2025 |
| DOI： | 10.1093/plphys/kiaf395 |
| Abstract： | Microalgae are a rich source of high-value natural products. The green microalga Chlamydomonas reinhardtii has long been used as a model organism for studying lipid metabolism in photosynthetic organisms. Here, we comprehensively characterized the enzymatic activity and substrate preferences of the plastidial glycerol-3-phosphate:acyl-CoA acyltransferase (GPAT1) from C. reinhardtii. Our results revealed that, in addition to GPAT activity, recombinant GPAT1 is associated with lysophosphatidic acid: acyl-CoA acyltransferase (LPAAT) activity. Notably, the membrane-bound form of GPAT1 displayed distinct acyl-donor preferences, favoring both C18:1 and C16:0 substrates in its LPAAT function. Knockdown of GPAT1 resulted in a reduced triacylglycerol content, particularly C16 species, under mixotrophic growth and nitrogen deprivation. Interestingly, GPAT1 knockdown triggered a compensatory upregulation of the endoplasmic reticulum-localized GPAT2, resulting in a significant increase in the content and yield of 1,3-olein-2-palmitin (OPO), an essential functional lipid used in infant formula. These findings provide insights into the function and physiological role of microalgal plastidial GPAT1 and highlight its potential as a biotechnological target for enhancing OPO production in microalgae. The plastid-localized enzyme GPAT1 in the microalga Chlamydomonas reinhardtii exhibits LPAAT activity in addition to its primary GPAT function. |
