Research
| Title： | Grass carp trim25 enhances rig-i/mda5-mediated antiviral innate immunity against GCRV infection |
|---|---|
| First author： | Li, Ziyi; Li, Zhi; Li, Jun; Song, Yanan; Yuan, Le; Wang, Yanyi; Yan, Runkun; Lai, Fuxiang; Xiao, Wuhan; Wang, Jing |
| Journal： | AQUACULTURE |
| Years： | 2025 |
| DOI： | 10.1016/j.aquaculture.2025.743087 |
| Abstract： | Grass carp (Ctenopharyngodon idella), which is farmed extensively throughout the world, has a key function in the aquaculture industry. But its haemorrhagic disease resulting from grass carp reovirus (GCRV) causes significant economic losses. Consequently, elucidating the GCRV tolerance mechanisms and identifying candidate genes for GCRV resistance are of great necessity. Here, we cloned the coding sequences (CDS) of grass carp tripartite motif containing 25 (gc-trim25) and sequence analysis revealed that gc-trim25 is conserved across species. GCRV infection induced gc-trim25 mRNA expression within most grass carp tissues and CIK cells. Additionally, gctrim25 enhanced interferon (IFN) activation that was induced by poly I:C-HMW and poly I:C-LMW. Furthermore, gc-trim25 enhanced antiviral gene expression and suppressed viral replication in an enzyme activitydependent manner upon infection with GCRV. Mechanistically, gc-trim25 interacts with retinoic acidinducible gene-i (rig-i) and melanoma-differentiation-associated gene-5 (mda5) for enhancing K63-linked gcrig-i and gc-mda5 ubiquitination, thus promoting the antiviral immunity. Furthermore, trim25-deficient zebrafish are more susceptible to GCRV infection. These findings help better understanding the effect of grass carp trim25 on antiviral innate immunity. |
