Research
| Title： | Characterization of type II IFNs and their receptors in a cyprinid fish, the blunt snout bream Megalobrama amblycephala |
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| First author： | Li, Bo; Chen, Shan Nan; Huang, Lin; Li, Li; Ren, Li; Hou, Jing; Tian, Jing Yun; Liu, Lan Hao; Nie, P. |
| Journal： | FISH & SHELLFISH IMMUNOLOGY |
| Years： | 2024 |
| DOI： | 10.1016/j.fsi.2024.109402 |
| Abstract： | Type II interferons (IFNs) are a key class of molecules regulating innate and adaptive immunity in vertebrates. In the present study, two members of the type II IFNs, IFN-gamma and IFN gamma-rel, were identified in the blunt snout bream (Megalobrama amblycephala). The open reading frame (ORF) of IFN-gamma and IFN gamma-rel was found to have 564 bp and 492 bp, encoding 187 and 163 amino acids, with the first 26 and 24 amino acids being the signal peptide, respectively. IFN-gamma and IFN gamma-rel genes showed a high degree of similarity to their zebrafish homologues, being 76.9 % and 58.9 %, respectively. In the phylogenetic tree, IFN-gamma and IFN gamma-rel were clustered with homologous genes in cyprinids. In blunt snout bream, IFN-gamma and IFN gamma-rel were constitutively expressed in trunk kidney, head kidney, spleen, liver, heart, muscle, gill, intestine and brain and were significantly up -regulated by poly (I:C) induction in head kidney, spleen, liver, gill and intestine. Using recombinant proteins of IFN-gamma and IFN gamma-rel, the surface plasmon resonance (SPR) results showed that IFN-gamma was bound to CRFB6, CRFB13 and CRFB17, but mainly to CRFB6 and CRFB13, whereas IFN-gamma rel bound mainly to CRFB17 and had no affinity with CRFB6. These results contribute to a better understanding on type II IFNs and their receptor usage in teleost fish. |
