Research
| Title： | Enhancing understanding of interferon-induced protein 35 (IFI35) in hybrid snakehead (Channa maculate 9 x Channa argus ♂): Cloning, expression, and interaction with EF1α in response to SHVV infection |
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| First author： | Liu, Xiaodan; Zhang, Yanbing; Tan, Xu; Zhang, Liwen; Guo, Mengya; Kong, Weiguang; Cao, Pan |
| Journal： | AQUACULTURE |
| Years： | 2024 |
| DOI： | 10.1016/j.aquaculture.2024.741222 |
| Abstract： | Interferon-induced protein 35 (IFI35) play a crucial role in the host's antiviral responses. However, research focusing on the characteristics of IFI35 genes in fish remains limited. In the present study, the IFI35 gene from hybrid snakehead was cloned and characterized. The potential interacting proteins of IFI35 to elucidate its role in disease resistance was also investigated. The open reading frame (ORF) of IFI35 was found to be 1119 bp, encoding a polypeptide with 372 amino acids. Sequence analysis revealed that the IFI35 protein in hybrid snakehead shares the highest identity with those in Micropterus salmoides and Siniperca chuatsi. Expression of the IFI35 gene was detected in all examined tissues of healthy fish, with the highest levels in the spleen, gills, and intestine. Under viral stress from snakehead vesicularvirus (SHVV), a significant induction of IFI35 expression was observed, especially in the liver and spleen. Yeast two-hybrid assays identified 21 proteins that interact with IFI35, including elongation factor 1-alpha (EF1 alpha), ribosomal proteins, transcription factors, collagen, ferritin-like domain, cytochrome c oxidase, and short chain dehydrogenase, etc. Subsequent validation confirmed the interaction between IFI35 and EF1 alpha by point-to-point validation. These findings suggest a significant role for IFI35 in the immune regulation of hybrid snakeheads, enhancing our understanding of its function and interaction networks, which could facilitate future explorations of IFI35 mechanisms in snakehead fish. |
