Research
| Title： | Fe2+inhibits spring viraemia of carp virus proliferation via ATG14-dependent autophagy |
|---|---|
| First author： | Li, Zhuo-Cong; Zhang, Can; Xu, Na; Xu, Xiao; Han, Ke-Jia; Zhou, Chu-Jing; Wang, Yang-Yang; Cui, Bao-Jie; Chen, Dan-Dan; Lu, Long-Feng; Li, Shun |
| Journal： | AQUACULTURE |
| Years： | 2025 |
| DOI： | 10.1016/j.aquaculture.2024.741858 |
| Abstract： | Iron (Fe) plays an essential role in diverse cellular activities, yet its participation in the context of fish virus infection remains elusive. In this work, we present findings on the antiviral efficacy of Fe2+ in instigating autophagic clearance of spring viraemia of carp virus (SVCV). In vivo, Fe2+ increases the survival rate of zebrafish infected with SVCV, mitigates pathological damage, and diminishes viral load in liver tissues. In vitro, Fe2+ impedes the proliferation of SVCV in cells. Further investigations reveal that Fe2+ stimulates cellular autophagy and induce autophagy post-viral infection, with the antiviral function of Fe2+ being compromised upon autophagy activation blockade. Mechanistically, the Fe2+-mediated clearance of SVCV virus relies on the pivotal autophagic initiation factor, autophagy related 14 (ATG14). This study elucidates the mechanism through which Fe2+ induces autophagic for viral clearance, underscoring the potential of low concentrations of exogenous iron as an efficacious therapeutic strategy for fish viral diseases. |
