Research
| Title： | Identification and function of type IV IFN in a cyprinid fish, the blunt snout bream Megalobrama amblycephala |
|---|---|
| First author： | Li, Bo; Liu, Zheng; Chen, Shan Nan; Wang, Kai Lun; Deng, Yu Hang; Pang, An Ning; Liu, Lan Hao; Zhang, Shan; Tian, Jing Yun; Ren, Li; Nie, P. |
| Journal： | AQUACULTURE |
| Years： | 2024 |
| DOI： | 10.1016/j.aquaculture.2024.741316 |
| Abstract： | Interferons (IFNs) represent a group of proteins with notable antiviral capabilities. Recently, the discovery of type IV IFN, i.e. IFN-o has created a new avenue for research on class II cytokines. In the present study, IFN-o was identified in blunt snout bream to possess conserved sequence CXXXXX[W/L] and gene locus. In the bream, IFNo was expressed in examined tissues and was induced significantly following poly(I:C) stimulation. With the stimulation of recombinant IFN-o in vivo, transcriptome was sequenced in head kidney, and 814 DEGs were identified with 165 possible IFN-stimulated genes (ISGs) characterized through comparison with type I IFN induced ISGs in zebrafish. Furthermore, IFN-o was found to show high affinity with CRFB12, but no interaction was observed between IFN-o and CRFB4, through surface plasmon resonance (SPR) and coimmunoprecipitation (Co-IP) analyses. These results should contribute to the understanding of IFN-o and its receptor complex, and importantly its immune function in inducing ISGs. |
