Research
| Title： | Parachitinimonas caeni gen. nov., sp. nov., a novel member of family Burkholderiaceae isolated from activated sludge collected in Shenzhen, PR China |
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| First author： | Wang, Xin; Chen, Han; Wang, Jing; Yuan, Siliang; Jiao, Dian; Wang, Shanhui; Liu, Yaqi; Li, Yan; Dai, Jingcheng; Li, Shuyang; Qiu, Dongru |
| Journal： | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| Years： | 2024 |
| DOI： | 10.1099/ijsem.0.006249 |
| Abstract： | A Gram- stain- negative, strictly aerobic and filamentous bacterial strain, designated as DQS- 5T, was isolated from the activated sludge of a municipal sewage treatment plant in Shenzhen, PR China. Optimal growth was observed at 28 degrees C and pH 7.5. Catalase and oxidase activities were detected. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DQS- 5T was most closely related to the genera Chitinimonas and Chitinivorax (91.0-93.4 % and 92.5 % 16S rRNA gene sequence similarity, respectively) and was close to the member of the family Burkholderiaceae. The complete genome sequence of strain DQS- 5T contains 5 653 844 bp and 57.3 mol% G+C. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between the genome of strain DQS- 5T and those of its close relatives were 75.9-77.2, 19.0-20.3 and 57.2- 61.8 %, respectively. Chemotaxonomic analysis of strain DQS- 5T indicated that the sole respiratory quinone was ubiquinone- 8, the predominant cellular fatty acids were C16: 0 and summed feature 3 (C16:1 omega 7c and/or C16:1 omega 6c), and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain DQS- 5T represents a novel species in a novel genus within the family Burkholderiaceae, for which the name Parachitinimonas caeni gen. nov., sp. nov., is proposed. Strain DQS- 5T (=KCTC 92788T=CCTCC AB 2022320T) is the type and only strain of P. caeni. |
