Research
| Title： | Single-chain antibody gene therapy strategy based on high-throughput screening triggers sustained antiviral activity in the body |
|---|---|
| First author： | Zhao, Liang; Wei, Xue-Feng; Xu, Kun; Zhao, Zhao; Chen, Guo; Wang, Hou-Peng; Zhu, Bin |
| Journal： | JOURNAL OF VIROLOGY |
| Years： | 2025 |
| DOI： | 10.1128/jvi.01497-24 |
| Abstract： | The occurrence of viral diseases poses a huge threat and impact on human public health safety and the development of the animal and fishery industry. Here, a strain of single-chain antibody fragment, scFv-1, was isolated from the phage antibody display library construct by immunizing New Zealand white rabbits with rhabdovirus. In vitro analysis showed that the single-chain antibody could inhibit the infection of the virus in multiple pathways, including adsorption, fusion, and release. In vivo analysis revealed scFv-1 had a preventive and protective effect against the infection of virus. In addition, we describe that transposon-based transport of neutralizing genes allows for long-term, continuous expression, avoiding the need for lifelong, repeated passive immunization for treatment. In sum, high-throughput screening of neutralization genes based on phage display technology and transposon vector-based gene transfer provides effective methods for treating and preventing diseases and avoiding repetitive passive immunotherapy. This study also provides a reference for the prevention and treatment of unknown pathogens. |
