Research
| Title： | The Zpr-3 Antibody Recognizes the 320-354 Region of Rho and Labels Both Rods and Green Cones in Zebrafish |
|---|---|
| First author： | Hu, Hualei; Qin, Yayun; Qu, Zhen; Huang, Yuwen; Ren, Xiang; Liu, Mugen; Liu, Fei; Gao, Pan |
| Journal： | ZEBRAFISH |
| Years： | 2024 |
| DOI： | 10.1089/zeb.2024.0159 |
| Abstract： | Retinal markers with high quality and specificity are important for the observation of pathologic changes of retinal cells during retinal development, degeneration, and regeneration. The zpr-3 antibody is widely used to label rods in zebrafish, but the exact antigen is still unknown. In this study, we provided evidence to demonstrate that the antigen gene of zpr-3 is rho, which encodes the rod opsin, and the exact epitope of zpr-3 is the 320-354 region of Rho protein. More importantly, our immunofluorescence assays indicated that zpr-3 labels both the outer segments of rods and green cones on zebrafish retinal sections, probably due to the cross-reaction with the green-cone opsin. Our work is valuable for the scientific community to interpret the experimental data involving the zpr-3 antibody. |
