Research
| Title： | miR-2188-5p promotes GCRV replication by the targeted degradation of klf2a in Ctenopharyngodon idellus |
|---|---|
| First author： | Li, Yangyu; Chen, Liangming; Li, Yangyang; Deng, Ping; Yang, Cheng; Li, Yongming; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping; Huang, Rong |
| Journal： | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
| Years： | 2023 |
| DOI： | 10.1016/j.dci.2022.104516 |
| Abstract： | Studies on host immunity evasion by aquatic viruses have largely focused on coding genes. There is accumulating evidence for the important biological functions of non-coding miRNAs in virus-host interactions. The regulatory functions of non-coding miRNAs in fish reovirus-host interactions remain unknown. Here, miR-2188-5p in grass carp (Ctenopharyngodon idellus), a miRNA specific to teleosts, was predicted to target the 3 ' UTR of the tran-scription factor klf2a. A correlation analysis and dual-luciferase reporter assay revealed that miR-2188-5p could induce the degradation of klf2a. The expression of miR-2188-5p induced the degradation of klf2a in a dose -dependent manner, suppressing the type I interferon response and promoting grass carp reovirus (GCRV) replication. As determined by a co-expression analysis, klf2a inhibited viral infection when miR-2188-5p was overexpressed. The targeted degradation of klf2a by miR-2188-5p could inhibit the type I interferon response and promote the replication of GCRV; however, this targeted degradation ability was insufficient to fully inhibit GCRV infection. These results provide novel insights into the regulatory effects and biological functions of non-coding miRNAs in fish-virus interactions. |
