Research
| Title： | Identification of type I IFNs and their receptors in a cyprinid fish, the topmouth culter Culter alburnus |
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| First author： | Li, Bo; Chen, Shan Nan; Ren, Li; Wang, Su; Liu, Lanhao; Liu, Yang; Liu, Shaojun; Nie, P. |
| Journal： | FISH & SHELLFISH IMMUNOLOGY |
| Years： | 2020 |
| DOI： | 10.1016/j.fsi.2020.04.069 |
| Abstract： | In fish, type I IFNs are classified into three groups, i.e. group one, group two and group three, and further separated into seven subgroups based on the number of conserved cysteines and phylogenetic relationships. In the present study, four type I IFNs, named as IFN phi 1, IFN phi 2, IFN phi 3, IFN phi 4, as reported in zebrafish, were identified in a cyprinid, the topmouth culter, Gaiter alburnus, a species introduced recently into China's aqua-culture. These IFNs may be classified as IFNa, IFNc, IFNc and IFNd in a recent nomenclature, with IFNa and IFNd having two cysteines in group one, and IFNc four cysteines in group two. These IFNs, together with their possible receptors, IFN phi 1, IFN phi 2, IFN phi 3, IFN phi 4, and CRFB1, CRFB2 and CRFB5 have an open reading frame (ORF) of 540, 552, 567, 516 bp, and 1572, 1392, 1125 bp, respectively. These IFNs have high amino acid sequence identities, being 91.1-93.6% and 66.9-77.3%, with those in grass carp and zebrafish, respectively, and are expressed constitutively in organs/tissues examined in the fish. The expression of these IFNs can be further induced following poly (I:C) stimulation. However, the possible function of these IFNs and their signalling pathway are of interest for further research. |
