Research
| Title： | In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-gamma, IRF1, and Two Forms of CIITA |
|---|---|
| First author： | Hou, Jing; Chen, Shan Nan; Gan, Zhen; Li, Nan; Huang, Lin; Huo, Hui Jun; Yang, Yue Cong; Lu, Yishan; Yin, Zhan; Nie, Pin |
| Journal： | JOURNAL OF IMMUNOLOGY |
| Years： | 2020 |
| DOI： | 10.4049/jimmunol.1801480 |
| Abstract： | Mammalian CIITA isoforms are tightly regulated by independent promoters. These promotors are induced by IFN-g through JAK-STAT signaling pathway. The induction of CIITA controls the expression of MHC class II (MHCII) and Ag presentation to the adaptive immune system. In the current study, to our knowledge, we first identified two independent promoters, p1 and p2, in the zebrafish ( Danio rerio) that control the expression of the two variants of CIITA, CIITA variant 1 (CIITAv1), and CIITA variant 2 (CIITAv2), respectively. Moreover, although IRF1 in an IFN-gamma signaling pathway induced CIITAv2, which has two ISRE motifs in its promoter, CIITAv1 expression was not induced by this signal. Further, the transcription of MHCII DAB was controlled by IRF1 via two distinct mechanisms: 1) the transcription of MHCII DAB was controlled by IRF1 indirectly through the two ISREs in p2; and 2) directly via the ISRE in MHCII DAB promoter. We also found that IRF1 associated with CIITAv1 and CIITAv2 via protein-protein interactions to synergistically drive the transcription of MHCII DAB. The IFN-gamma-IRF1-CIITA-MHCII signaling cascade was functional in early life stages of CIITA(-/-) and IRF1(-/-) zebrafish. Our findings imply that the immune system develops early in fishes and that the IFN-g signaling cascade-induced CIITA and MHCII DAB is conserved in teleost fishes and mammals. |
