Research
| Title： | Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtgl) mRNA expression |
|---|---|
| First author： | Chen, Minjie; Zhang, Jie; Pang, Shaochen; Wang, Chang; Wang, Ling; Sun, Yonghua; Song, Maoyong; Liang, Yong |
| Journal： | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY |
| Years： | 2018 |
| DOI： | 10.1016/j.cbpc.2017.11.010 |
| Abstract： | By measuring the vitellogenin 1 (vtgl) expression through quantitative PCR and in situ hybridization, we used the zebrafish embryo as an in vivo model to access the estrogenic or anti-estrogenic effects of several endocrine disrupting chemicals (EDCs), such as natural estrogen 17 beta-estradiol (E2), estriol (E3), synthetic hormones including diethylstilbestrol (DES), 4-octyl phenol (OP), bisphenol A (BPA), tamoxifen (TMX) and 3-(2,3-dibromopropyl) isocyanurate (TBC). According to our data, the estrogenic effect Of the tested chemicals was ranked as: DES > E-2 > E-3 > OP > BPA, which is consistent with various in vivo and in vitro models. Therefore, the measurement of vtgl gene expression in zebrafish embryos would be a valuable method for screening EDCs including both environmental estrogens and anti-estrogens. |
