Research
| Title： | Processing of PatS, a morphogen precursor, in cell extracts of Anabaena sp PCC 7120 |
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| First author： | Zhang, Lianglin; Zhou, Fang; Wang, Shuai; Xu, Xudong |
| Journal： | FEBS LETTERS |
| Years： | 2017 |
| DOI： | 10.1002/1873-3468.12582 |
| Abstract： | Upon N-stepdown, Anabaena sp. PCC 7120 differentiates heterocysts along filaments in a semiregular pattern. A 17-amino acid peptide called PatS is a morphogen precursor for pattern formation. The principal PatS derivative involved in heterocyst patterning has been proposed to be the C-terminal peptide PatS-5 (RGSGR), PatS-6 (ERGSGR), or PatS-8 (CDERGSGR). We present the first evidence for processing of PatS in cell extracts of this cyanobacterium. PatS is probably cleaved between the C-terminal 7th and 8th amino acid residues, producing PatS-7 (DERGSGR), then converted into PatS-6 and PatS-5. The processing site could be changed by a substitution at the C-terminal 8th residue. |
