Research
| Title： | Preferential combination between the light and heavy chain isotypes of fish immunoglobulins |
|---|---|
| First author： | Zhang, Nu; Zhang, Xu-Jie; Song, Yu-Long; Lu, Xiao-Bing; Chen, Dan-Dan; Xia, Xiao-Qin; Sunyer, J. Oriol; Zhang, Yong-An |
| Journal： | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
| Years： | 2016 |
| DOI： | 10.1016/j.dci.2016.04.001 |
| Abstract： | Immunoglobulin light chain (IgL) is necessary for the assembly of an Ig molecule, which plays important roles in the immune response. IgL genes were identified in various teleost species, but the basic functions of different IgL isotypes and the preferential combination between IgL and IgH (Ig heavy chain) isotypes remain unclear. In the current study, by EST database searching and cDNA cloning in rainbow trout, 8 IgL sequences were obtained, which could be classified into the IgL kappa F, IgL kappa G, IgL sigma and IgL lambda isotypes, respectively. Trout IgL isotypes were highly expressed in the immune-related tissues, and participated in the immune responses in spleen and gut by stimulation with LPS and poly (I:C). The results of FACS and LC-MS/MS indicated that the IgL kappa G and IgL sigma isotypes preferentially bonded with the heavy chains of IgM and IgT, respectively, in trout B cells and serum. In addition, the genomic organization of trout IgL isotypes and the utilization of recombination signal sequences were studied. (C) 2016 Elsevier Ltd. All rights reserved. |
