Research
| Title： | Extracellular Polymeric Substances of Aphanizomenon flos-aquae (EPS-A) Induced Apoptosis in Astrocytes of Zebrafish |
|---|---|
| First author： | Lv, Ying; Xue, Xing; Tao, Ling; Zhang, Delu; Hu, Chunxiang; Ren, Jun |
| Journal： | BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY |
| Years： | 2016 |
| DOI： | 10.1007/s00128-016-1729-3 |
| Abstract： | In this study, extracellular polymeric substances of Aphanizomenon flos-aquae (EPS-A) were investigated in order to explore their effect on astrocytes of zebrafish and potential risk for environment. Astrocytes were treated with varying concentrations of EPS-A, the results showed that EPS-A inhibited astrocytes growth in a dose-and time-dependent manner. With the concentrations of EPS-A increasing, the adherent ability of astrocytes decreased and the number of astrocytes floating in the culture medium increased. When treated with 2.35 A mu g/mL EPS-A, EPS-A induced cell cycle arrest and made the collapse of mitochondrial membrane potential and then led to astrocytes apoptosis. The results suggested that EPS-A could pose a threat to zebrafish and represent risk for environment, so regularly monitoring the presence of EPS-A was very important in nutrient-rich freshwaters when A. flos-aquae blooms broke out. |
